Part:BBa_K1179008

A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

This plasmid contains the coding sequence for a DNA binding non-nuclease mutant of Cas9 followed by a linker sequence and a BsaI site for ligation of a protein of interest to the C-terminal end of the Cas9. When expressed with a guide RNA, the Cas9 will target the location as dictated by the guide RNA and localize your fused protein to that region.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4243
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4243
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4243
  Illegal BglII site found at 295
  Illegal BglII site found at 1232
  Illegal BamHI site found at 1
  Illegal BamHI site found at 2050
  Illegal XhoI site found at 3588
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4243
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4243
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  Illegal NgoMIV site found at 2784
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4219
  Illegal BsaI.rc site found at 4210